Instructions:

Using the Graphical Display:

  1. Select an enzyme system using the pull-down menu to load default inputs. If desired, the values inside text edit boxes can be changed by hand. To avoid execution and overflow errors it is recommended to start using default inputs.

  2. Click the "Run Simulation" button. The simulation returns two types of results, a Rate of Reaction (inside the yellow box) and plots showing the time evolution of chemical species, such as substrate, enzyme product, inhibitor and complexes.

  3. To add or remove plots from the plot panel check "Plot Controls" boxes, and click the Run Simulation button again.

Definitions:

Plot Controls
S: Substrate. A molecule upon which an enzyme acts.
E: Enzyme. A chemical reaction catalyst.
ES: Enzyme/substrate complex. A chemical species formed by non-covalent binding of substrate to enzyme.
P: Product. A chemical species "manufactured" by an enzyme from its substrate.
I: Inhibitor. A chemical species that reduces the catalytic efficiency of an enzyme.
EI: Inhibitor/enzyme complex. A chemical species formed by non-covalent binding of inhibitor to enzyme.
ESI: Inhibitor/substrate/enzyme complex. A chemical species formed by non-covalent binding of inhibitor to the enzyme/substrate complex.